Lipidomics Mixtures and Kits

CIL offers a number of multi-component mixtures for lipidomics research. The standards in the mixes are uniformly (e.g., U-13C mixed fatty acids) or partially (e.g., lysophosphatidylcholine or LysoPC mix) labeled and are available in research and/or microbiological/pyrogen tested (MPT) grades. Crude lipid yeast extracts (U-13C and unlabeled) are also available for use as standards in MS-based lipidomics studies. The fatty acids and lipids comprising these extracts span broad classes/pathways and are involved in a myriad of cellular/molecular processes. The extract kits are accompanied with a user manual, which provides a guide to the preparation and processing possibilities for product implementation, as well as supplying further resources for additional reference.

Related Resouces

Stable Isotopes for Lipidomics

Related Products

Frequently Asked Questions

What is supplied with a lipid yeast extract kit? Supplied is one vial of a U-13C or unlabeled lipid yeast extract and a document package (user manual, CoA, SDS, and product flyer). The user manual contains solution preparation and application example methods along with example analytical results (e.g., from HILIC- and RPLC-MRM/MS). Parameter tables for profiling and targeted MS analysis on example instrument platforms are also provided therein.

What is a suitable procedure for dissolving the lipid yeast extracts? An example reconstitution approach involves dissolving the extract in 1 mL solvent (e.g., isopropanol, chloroform) followed by a high-speed vortex and a brief centrifuge. This results in a clear solution.

What classes of lipids have been measured in the lipid yeast extracts? Measurable classes include glycerolipids (e.g., diglyceride, triglyercide), glycerophospholipids (e.g., phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine), and lysophospholipids (e.g., lysophosphatidylcholine, lysophosphatidylethanolamine). Acylcarnitines, cardiolipins, coenzymes, and fatty acids have also been reproducibly measured using MS-based methods. Please refer to CIL's Stable Isotope-Labeled Mixtures, Sets, and Kits catalog for a list of the reproducibly measured fatty acids and lipids.

What algal strain is used to create the mixed fatty acid and triglyceride products? Agmenelum quadriplicatum

Example References

Xie, J.; Bruggeman, A.; De Nolf, C.; et al. 2023. Gut microbiota regulates blood-cerebrospinal fluid barrier function and Aβ pathology. EMBO J, 42(17), e111515-3111533. PMID: 37427561
Xia, F.; Guo, L.; Cui, P.; et al. 2023. A sensitive and accurate GC-MS method for analyzing microbial metabolites short chain fatty acids and their hydroxylated derivatives in newborn fecal samples. J Pharm Biomed Anal, 223, 115148-115152.
PMID: 36403347
Zeng, X.; Xing, X.; Gupta, M.; et al. 2022. Gut bacterial nutrient preferences quantified in vivo. Cell, 185(18), 3441-3456.
PMID: 36055202
Chen, J.; Zou, L.; Lu, G.; et al. 2022. PFKP alleviates glucose starvation-induced metabolic stress in lung cancer cells via AMPK-ACC2 dependent fatty acid oxidation. Cell Discov, 8(1), 52-67. PMID: 
Bihan, D.G.; Rydzak, T.; Wyss, M.; et al. 2022. Method for absolute quantification of short chain fatty acids via reverse phase chromatography mass spectrometry. PLoS One, 17(4), e0267093-0267106. PMID: 35443015
Hernandez-Saavedra, D.; Sanders, L.; Freeman, S.; et al. 2020. Stable isotope metabolomics of pulmonary artery smooth muscle and endothelial cells in pulmonary hypertension and with TGF-beta treatment. Sci Rep, 10(1), 413-425. PMID: 31942023
He, C.; Weston, T.A.; Jung, R.S.; et al. 2018. NanoSIMS analysis of intravascular lipolysis and lipid movement across capillaries and into cardiomyocytes. Cell Metab, 27(5), 1055-1066. 
PMID: 29719224