QC and Quantitation Kits

PeptiQuant™ Plus QC Kits

The QC kits are used to test the effectiveness of a bottom-up proteomic method (workflow QC kit) or a LC-MS platform (daily QC kit). The workflow kit contains five components (i.e., stable isotope-labeled peptide mix, unlabeled peptide mix, trypsin, BSA, and human plasma), while the daily QC kit contains one component (i.e., stable isotope-labeled peptide mixture). The unit sizes are run numbers (workflow QC kit) or injection numbers (daily QC kit). To help with the execution is a detailed user manual and method files. For additional information, please refer to the Peptiquant Plus Quality Control Kits flyer.

PeptiQuant™ Plus BAKs

The BAKs are designed for the targeted and precise quantitation of ≥125 candidate disease protein biomarkers in human or mouse plasma samples. Each kit contains two synthetic peptide mixtures (stable isotope-labeled, SIS; unlabeled, NAT), trypsin, and BSA. The NAT peptide mixture is used to prepare the standard curve and curve QCs (both prepared in surrogate BSA/PBS matrix). The SIS peptide mixture is used in the standard curve and curve QCs as well as spike-in standards for the experimental samples. To help with the execution is a detailed user manual and method files. For additional information, please refer to the PeptiQuant™ Plus Biomarker Assessment Kits flyer.

❛❛The PeptiQuant Plus Platform Performance Kit has proven to be a vital component of our everyday quality assurance that enables us to deliver high-quality targeted proteomics data in an accurate and timely manner. This kit has a ‘dilute and shoot’ operation and comes with vendor-specific LC-MRM/MS parameters and a Skyline analysis file for quick input and results output. Altogether, the performance kit is an excellent means to rapidly assess LC-MS performance that should become a routine staple in a proteomic user’s toolbox.❜❜

– Tasso Miliotis, PhD | Associate Principal Scientist at AstraZeneca Gothenburg (Sweden)

Frequently Asked Questions

How many peptides/protein and MRM transitions do the PeptiQuant Plus kits monitor? At least one peptide/protein and 3 transitions/peptide (for MRM-based platforms) or 3 precursor ions/peptide (for PRM-based platforms).

What types of calibration curves can be prepared with the PeptiQuant Plus BAKs? The SIS and NAT peptide mixes enable eight-point curves that span a 1000-fold concentration range. Each calibrant level is to be measured in singleton.

What are the contents of the PeptiQuant Plus kits? Supplied with the kits are chemicals/reagents and a USB of product documents. Please see the QC kit and BAK flyers for details (see Related Resources above).

Are the PeptiQuant Plus kits platform dependent? The kits have been optimized on specific LC-MS platforms (see QC kit and BAK flyers for details). The validated methods are provided with the kits in an instrument file, while the transition (in MRM analysis) or precursor ion (in PRM analysis) details are provided in a Skyline file for data analysis. These files are supplied, together with the user manual, on a USB along with the chemicals/reagents. Note that while the kits are optimized for specific LC-MS platforms, the kits are extendable to other platforms following minor parameter optimizations.

What types of disease association have the proteins in the human and mouse plasma PeptiQuant Plus BAKs been linked to? The proteins have been linked to innumerable diseases (e.g., cancer, cardiovascular, neurodegenerative). Specific linkages can be provided upon request.

Are the 125 proteins in the human plasma BAK-125 contained in the human plasma BAK-270? Yes. The BAK-270 is complemented by an additional 145 proteins of moderate-to-low abundance. Please see the PeptiQuant Plus Biomarker Assessment Kits flyer for panel details.

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