Enzymatic Labeling

The incorporation of 18O atoms into a C-terminus of peptides has emerged as one of the labeling strategies used in comparative quantitative proteomics. The success of this enzymatic labeling technique is due in part to the relative low cost of 18O water, the resulting mass increase in molecular weight for a “heavy” peptide, and the co-elution of 18O/16O peptide pairs in a liquid chromatography (LC)-based mass spectrometry (MS) experiment.

Related Resources

Stable Isotope Standards for Mass Spectrometry

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Example References

Westereng, B.; Arntzen, M.O; Ostby, H.; et al. 2023. Analyzing activities of lytic polysaccharide monooxygenases by liquid chromatography and mass spectrometry. Methods Mol Biol, 2657, 27-51. PMID: 37149521 
Rumiantseva, L.; Osipenko, S.; Podolskiy, I.I.; et al. 2022. Increasing the reliability of compound identification in biological samples using 16O/18O-exchange mass spectrometry. Anal Bioanal Chem, 414(8), 2537-2543. PMID: 35103806 
Cho, Y.K.; Son, Y: ASaha, A.; et al. 2021. STK3/STK4 signalling in adipocytes regulates mitophagy and energy expenditure. Nat Metal, 3(3), 428-441. PMID: 33758424
Farthing D.E.; Buxbaum, N.P.; Lucas, P.J.; et al. 2017. Comparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water. Sci Rep, 7(1), 4043. PMID: 28642474 
Liu, M.; Zhang, Z.; Cheetham, J.; et al. 2017. Discovery and characterization of a photo-oxidative histidine-histidine cross-link in IgG1 antibody utilizing ¹⁸O-labeling and mass spectrometry. Anal Chem, 86(10), 4940-4948. PMID: 24738698 
Liu, M.; Zhang, Z., Zang, T.; et al. 2013. Discovery of undefined protein cross-linking chemistry: a comprehensive methodology utilizing 18O-labeling and mass spectrometry. Anal Chem, 85(12), 5900-5908. PMID: 23634697 
Auclair, J.R.; Somasundaran, M.; Green, K.M., et al. 2012. Mass spectrometry tools for analysis of intermolecular interactions. Methods Mol Biol, 896, 387-398. PMID: 22821539 
Fenselau, C.; Yao, X. 2009. 18O2-labeling in quantitative proteomic strategies: a status report. J Proteome Res, 8(5), 2140-2143. PMID: 19338309