脂质组学标准

CIL 为脂质组学研究提供多样化的稳定同位素标记和未标记脂肪酸/脂质库。这些标签有各种标签模式(即统一、部分)、形式(即游离酸、盐、酯)和材料等级(即研究、微生物/热原测试 - MPT)。脂肪酸涵盖饱和(例如,肉豆蔻酸、棕榈酸、硬脂酸)和不饱和(例如,棕榈油酸、亚油酸、油酸)类别。脂质包括神经酰胺和磷脂/溶血磷脂以及三酰基甘油酯和肉碱/酰基肉碱。这些同位素标记的化合物可用于生物样品中脂肪酸/脂质的通量测定和/或相对或绝对定量。

相关资源

Stable Isotopes for Lipidomics

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常见问题

溶解氘代脂肪酸 DHA 和 EPA 的合适溶剂是什么? 氘代 DHA(例如 D5 猫号 DLM-10012) 和 EPA(例如,D5 目录号。DLM-9720) 已被证明是有效的 溶于甲醇或乙醇。

大量 CIL 脂肪酸/脂质带有 13C 标记。 13C 标记与 D 标记在 MS 测量中有何优势?与 D 标记相比,13C 标记可以提供改进的结果 同位素稳定性、可忽略的同位素扰乱问题、保守的色谱洗脱(相对于其未标记的标准品,以及更高的分析可靠性。

参考实例

Weiss, J.M.; Palmieri, E.M.; Gonzalez-Cotto, M.; et al. 2023. Itaconic acid underpins hepatocyte lipid metabolism in non-alcoholic fatty liver disease in male mice. Nat Metab, 5(6), 981-995.PMID: 37308721
Klievik, B.J.; Metherel, A.H.; Cisbani, G.; et al. 2023. Novel 13C enrichment technique reveals early turnover of DHA in peripheral tissues. J Lipid Res, 64(5), 100357-100366. PMID: 36948271
Muhammad, N.; Ruiz, F.; Stanley, J.; et al. 2022. Monounsaturated and diunsaturated fatty acids sensitize cervical cancer to radiation therapy. Cancer Res, 82(24), 4515-4527.PMID: 36214635
Watkins, O.C.; Selvam, P.; Pillai, R.A.; et al. 2022. Myo-inositol moderates maternal BMI and glycemia related variations in in-vitro placental 13C-DHA-metabolism, altering their relationships with birthweight. Sci Rep, 12(1), 14895-14908. PMID: 36050341
da Silva, K.M.; Iturrospe, E.; van den Boom, R.; et al. 2022. Lipidomics profiling of zebrafish liver through untargeted liquid chromatography-high resolution mass spectrometry. J Sep Sci, 45(15), 2935-2945. PMID: 35716100
Chen, J.; Zou, L.; Lu, G.; et al. 2022. PFKP alleviates glucose starvation-induced metabolic stress in lung cancer cells via AMPK-ACC2 dependent fatty acid oxidation. Cell Discov, 8(1), 52-67. PMID: 35641476
Andersen, J.V.; Westi, E.W.; Jakobsen, E.; et al. 2021. Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply. Mol Brain, 14(1), 132-144. PMID: 34479615
Aidaros, A.A.; Sharma, C.; Langhans, C-D.; et al. 2020. Targeted metabolomic profiling of total fatty acids in human plasma by liquid chromatography-tandem mass spectrometry. Metabolites, 10(10), 400-414. PMID: 33050140
Hui, S,; Cowan, A.J.; Zeng, X.; et al. 2020. Quantitative fluxomics of circulating metabolites. Cell Metab, 32(4), 676-688. PMID: 32791100
Choi,H.-J.; Jhe, Y.-L.; Kim, J.; et al. 2020. FoxM1-dependent and fatty acid oxidation-mediated ROS modulation is a cell-intrinsic drug resistance mechanism in cancer stem-like cells. Redox Bill, 36, 101589-101588. PMID: 32521504 
He, C.; Weston, T.A.; Jung, R.S.; et al. 2018. NanoSIMS analysis of intravascular lipolysis and lipid movement across capillaries and into cardiomyocytes. Cell Metab, 27(5), 1055-1066. PMID: 29719224