代谢

研究人员采用稳定同位素技术来研究各种代谢紊乱和疾病，包括阿尔茨海默病、帕金森病、癌症、糖尿病和肥胖症。 同位素最常用于代谢研究作为示踪剂来量化生化 或体内代谢反应。 它们可用于研究代谢途径、确定生物标志物、测试药物的效果以及开发特定状态下生物系统的代谢特征。

产品等级

CIL 为我们的许多产品提供额外的测试，作为向客户提供的服务。 以下文档描述了这些产品的性质、对其应用的不同控制级别以及增强的技术数据包 适用于某些产品。

Stable Isotope-Labeled Products for Metabolic Research 

下载目录

相关资源

➤ Stable Isotope Standards for Mass Spectrometry
➤ Cancer Metabolism and Related Research
➤ Amino Acid Indicators and Protein Turnover
➤ Stable Isotope Tracing in Cancer Metabolism
➤ The Impact of Stable Isotope Tracers on Metabolic Research 
➤ Research Use of CIL Products
➤ Product Quality Designations
➤ Enhanced Data Package (EDP)

Application Notes
➤ Pathway-Targeted Metabolomic Analysis in Oral/Head and Neck Cancer Cells Using Ion Chromatography-Mass Spectrometry
 ➤ Analysis of Whole-Body Branched-Chain Amino Acid Metabolism in Mice Utilizing 20% Leucine 13C6 and 20% Valine 13C6 Mouse Feed 
➤ Fluxing Through Cancer: Tracking the Fate of 13C-Labeled Energy Sources Glucose and Glutamine in Cancer Cells and Mouse Tumors

相关产品

Amino Acids view all

Carbohydrates view all

Steroids and Hormones view all

Fatty Acids view all

Other Tracers

MRI/MRS view all

Hyperpolarization

13C Probes view all

Deuterated Metabolic Imaging (DMI)

Water view all

经常问的问题

CIL 的 -MPT 产品何时进行微生物含量测试？ 它们在发布时以散装形式进行测试。 收到订单后，后续等分样品不会重新测试和保证。 微生物测试并不意味着适合任何预期用途。

微生物检测适用于哪些生物？ –MPT 产品经过金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、沙门氏菌、需氧菌、酵母、霉菌和细菌内毒素测试。

微生物检测的限度是多少？ 对于大多数产品，需氧细菌、酵母和霉菌的限值为 <10 cfu/g。 这些产品还“通过”金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、沙门氏菌。

内毒素检测 (LAL) 的限值是多少？ 大多数产品的 LAL 规格为 <0.25 EU/mg，但有些产品可能有所不同（<0.125、<0.03、<0.01 等）。 实际的 LAL 结果报告在特定批次的 CoA 上。

CIL 能否对研究级（-0 产品）或微生物测试材料（-MPT）进行额外测试？ 是的，CIL 能够对大多数产品执行额外测试。 订购前应对此进行审查和报价。 可能需要支付额外费用。

CIL 是否提供用于临床试验的产品？ 是的，CIL 可以生产适合临床试验的 cGMP 级材料。 请联系我们讨论您的项目。

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参考实例

Meiser, J.; Tumanov, S.; Maddocks, O.; et al. 2016. Serine one-carbon catabolism with formate overflow. Sci Adv, 2(10), e1601273. PMID: 27819051
Svensson, R.U.; Parker, S.J.; Eichner, L.J.; et al. 2016. Inhibition of acetyl-coa carboxylase suppresses fatty acid synthesis and tumor growth of non-small-cell lung cancer in preclinical models. Nat Med, 22(10), 1108-1119.  PMID: 27643638
Mayers, J.R.; Torrence, M.E.; Danai, L.V.; et al. 2016. Tissue of origin dictates branched-chain amino acid metabolism in mutant KRAS-driven cancers. Science, 353(6304), 1161-1165. PMID: 27609895
Engelen, M.P.; Safar, A.M.; Bartter, T.; et al. 2016. Reduced arginine availability and nitric oxide synthesis in cancer is related to impaired endogenous arginine synthesis. Clin Sci, 130(14), 1185-1195.  PMID: 27129191
Sellers, K.; Fox, M.P.; Bousamra, M. 2nd.; et al. 2015. Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation. J Clin Invest, 125(2), 687-698. PMID: 25607840
Green, C.O.; Badaloo, A.V.; Hsu, J.W.; et al. 2014. Effects of randomized supplementation of methionine or alanine on cysteine and glutathione production during the early phase of treatment of children with edematous malnutrition. Am J Clin Nutr, 99(5), 1052-1058.  PMID: 24598154
Nelson, S.J.; Kurhanewicz, J.; Vigneron, D.B.; et al. 2013. Metabolic imaging of patients with prostate cancer using hyperpolarized [1-13C]pyruvate. Sci Transl Med, 5(198).  PMID: 23946197
de Betue, C.; Joosten, K.; Deutz, N.; et al. 2013. Arginine appearance and nitric oxide synthesis in critically ill infants can be increased with a protein-energy-enriched enteral formula. Am J Clin Nutr, 98(4), 907-916.  PMID: 23945723
Alauddin, M. 2012. Positron emission tomography (pet) imaging with 18F-based radiotracers. Am J Nucl Med Mol Imaging. 2012;2(1), 55-76. PMID: 23133802
Elango, R.; Ball, R.O.; Pencharz, P.B. 2012. Recent advances in determining protein and amino acid requirements in humans. Br J Nutr, 108.  PMID: 23107531
Son, J.; Lyssiotis, C.A.; Ying, H.; et al. 2013. Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway. Nature, 496(7443), 101-105.  nature.com/articles/nature12040.
Maher, E.A.; Marin-Valencia, I.; Bachoo, et al. 2012. Metabolism of [U-13C]glucose in human brain tumors in vivo. NMR Biomed, 25(11), 1234-1244.  PMID: 22419606
Dunn, W.B.; Broadhurst, D.; Begley, P.; et al. 2011. Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nat Protoc, 6(7), 1060-1083.  PMID: 21720319
Henderson, G.C.; Dhatariya, K.; Ford, G.C.; et al. 2009. Higher muscle protein synthesis in women than men across the lifespan, and failure of androgen administration to amend age-related decrements. FASEB J, 23(2), 631-641.  PMID: 18827019
Jaleel, A.; Nehra, V.; Persson, X.M.; et al. 2007. In vivo measurement of synthesis rate of multiple plasma proteins in humans. Am J Physiol Endocrinol Metab, 292(1), E190-197.  PMID: 16449301
Previs, S.F.; Fatica, R.; Chandramouli, V.; et al. 2004. Quantifying rates of protein synthesis in humans by use of 2H2O: Application to patients with end-stage renal disease. Am J Physiol Endocrinol Metab, 286(4), E665-672.  PMID: 14693509
Cobelli, C.; Saccomani, M.P.; Tessari, P.; et al. 1991. Compartmental model of leucine kinetics in humans. Am J Physiol, 261, E539-550.  PMID: 1928344
Picou, D.; Taylor-Roberts, T. 1969. The measurement of total protein synthesis and catabolism and nitrogen turnover in infants in different nutritional states and receiving different amounts of dietary protein. Clin Sci, 36(2),283-296.   PMID: 5772104