Lipidomics Standards

CIL offers a diverse library of stable isotope-labeled and unlabeled fatty acids/lipids for lipidomics research. These are available in various labeling patterns (i.e., uniform, partial), forms (i.e., free acid, salt, ester), and material grades (i.e., research, microbiological/pyrogen tested – MPT). The fatty acids cover saturated (e.g., myristic, palmitic, stearic) and unsaturated (e.g., palmitoleic, linoleic, oleic) classes. The lipids include ceramides and phospholipids/lysophospholipids as well as triacylglycerides and carnitine/acylcarnitines. These isotopically labeled compounds can be used for flux determination and/or relative or absolute quantification of fatty acids/lipids in biological samples.

➤ Stable Isotope Standards for Mass Spectrometry
➤ Stable Isotope-Labeled Products for Metabolic Research
➤ Small Package Sizes for Key Metabolite Standards

Stable Isotopes for Lipidomics

Frequently Asked Questions

What is an appropriate solvent for dissolving the deuterated fatty acids DHA and EPA? Deuterated DHA (e.g., D₅ catalog no. DLM-10012) and EPA (e.g., D₅ catalog no. DLM-9720) have demonstrated to be effectively solubilized in methanol or ethanol.

A large number of CILs fatty acids/lipids are ¹³C-labeled. What are the advantages of ¹³C labeling vs. D labeling in MS measurements? In comparison to D labels, ¹³C labels can provide improved isotope stability, negligible isotope scrambling issues, conserved chromatographic elution (relative to its unlabeled standard), and heightened analytical reliability.

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Klievik, B.J.; Metherel, A.H.; Cisbani, G.; et al. 2023. Novel ¹³C enrichment technique reveals early turnover of DHA in peripheral tissues. J Lipid Res, 64(5), 100357-100366. PMID: 36948271
Muhammad, N.; Ruiz, F.; Stanley, J.; et al. 2022. Monounsaturated and diunsaturated fatty acids sensitize cervical cancer to radiation therapy. Cancer Res, 82(24), 4515-4527.PMID: 36214635
Watkins, O.C.; Selvam, P.; Pillai, R.A.; et al. 2022. Myo-inositol moderates maternal BMI and glycemia related variations in in-vitro placental ¹³C-DHA-metabolism, altering their relationships with birthweight. Sci Rep, 12(1), 14895-14908. PMID: 36050341
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Chen, J.; Zou, L.; Lu, G.; et al. 2022. PFKP alleviates glucose starvation-induced metabolic stress in lung cancer cells via AMPK-ACC2 dependent fatty acid oxidation. Cell Discov, 8(1), 52-67. PMID: 35641476
Andersen, J.V.; Westi, E.W.; Jakobsen, E.; et al. 2021. Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply. Mol Brain, 14(1), 132-144. PMID: 34479615
Aidaros, A.A.; Sharma, C.; Langhans, C-D.; et al. 2020. Targeted metabolomic profiling of total fatty acids in human plasma by liquid chromatography-tandem mass spectrometry. Metabolites, 10(10), 400-414. PMID: 33050140
Hui, S,; Cowan, A.J.; Zeng, X.; et al. 2020. Quantitative fluxomics of circulating metabolites. Cell Metab, 32(4), 676-688. PMID: 32791100
Choi,H.-J.; Jhe, Y.-L.; Kim, J.; et al. 2020. FoxM1-dependent and fatty acid oxidation-mediated ROS modulation is a cell-intrinsic drug resistance mechanism in cancer stem-like cells. Redox Bill, 36, 101589-101588. PMID: 32521504 
He, C.; Weston, T.A.; Jung, R.S.; et al. 2018. NanoSIMS analysis of intravascular lipolysis and lipid movement across capillaries and into cardiomyocytes. Cell Metab, 27(5), 1055-1066. PMID: 29719224