Lipidomics Mixtures and Kits

CIL offers a number of multi-component mixtures for lipidomics research. The standards in the mixes are uniformly (e.g., U-¹³C mixed fatty acids) or partially (e.g., lysophosphatidylcholine or LysoPC mix) labeled and are available in research and/or microbiological/pyrogen tested (MPT) grades. Crude lipid yeast extracts (U-¹³C and unlabeled) are also available for use as standards in MS-based lipidomics studies. The fatty acids and lipids comprising these extracts span broad classes/pathways and are involved in a myriad of cellular/molecular processes. The extract kits are accompanied with a user manual, which provides a guide to the preparation and processing possibilities for product implementation, as well as supplying further resources for additional reference.

➤ Stable Isotope Standards for Mass Spectrometry
➤ Stable Isotope-Labeled Products for Metabolic Research
➤ Stable Isotope-Labeled Mixtures, Sets, and Kits
➤ Mixtures, Sets, and Kits for MS 'Omics and MS/MS Screening

Application Note
➤ Targeted MRM Screening of U-¹³C Lipid Yeast Extracts for Robust Lipidomics Applications

Stable Isotopes for Lipidomics

Frequently Asked Questions

What is supplied with a lipid yeast extract kit? Supplied is one vial of a U-¹³C or unlabeled lipid yeast extract and a document package (user manual, CoA, SDS, and product flyer). The user manual contains solution preparation and application example methods along with example analytical results (e.g., from HILIC- and RPLC-MRM/MS). Parameter tables for profiling and targeted MS analysis on example instrument platforms are also provided therein.

What is a suitable procedure for dissolving the lipid yeast extracts? An example reconstitution approach involves dissolving the extract in 1 mL solvent (e.g., isopropanol, chloroform) followed by a high-speed vortex and a brief centrifuge. This results in a clear solution.

What classes of lipids have been measured in the lipid yeast extracts? Measurable classes include glycerolipids (e.g., diglyceride, triglyercide), glycerophospholipids (e.g., phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine), and lysophospholipids (e.g., lysophosphatidylcholine, lysophosphatidylethanolamine). Acylcarnitines, cardiolipins, coenzymes, and fatty acids have also been reproducibly measured using MS-based methods. Please refer to CIL's Stable Isotope-Labeled Mixtures, Sets, and Kits catalog for a list of the reproducibly measured fatty acids and lipids.

What algal strain is used to create the mixed fatty acid and triglyceride products? Agmenelum quadriplicatum

Chen, J.; Zou, L.; Lu, G.; et al. 2022. PFKP alleviates glucose starvation-induced metabolic stress in lung cancer cells via AMPK-ACC2 dependent fatty acid oxidation. Cell Discov, (1), 52-77. PMID: 35641476
Hernandez-Saavedra, D.; Sanders, L.; Freeman, S.; et al. 2020. Stable isotope metabolomics of pulmonary artery smooth muscle and endothelial cells in pulmonary hypertension and with TGF-beta treatment. Sci Rep, 10(1), 413-425. PMID: 31942023
He, C.; Weston, T.A.; Jung, R.S.; et al. 2018. NanoSIMS analysis of intravascular lipolysis and lipid movement across capillaries and into cardiomyocytes. Cell Metab, 27(5), 1055-1066.  PMID: 29719224