Chemical Tagging

Metabolic incorporation of heavy isotopes into a proteome, as in SILAC and SILAM, is a popular method to prepare an internal standard or labeled control; however, some organisms and animals are not amenable to metabolic incorporation. Fortunately, analytes may be readily modified through chemical tagging reactions. Examples include the reductive animation of primary amines in proteins or peptides and hydrazide tagging of glycans in proteomic samples. Since tagging reagents are compatible with many biological sample types, CIL offers a collection of reductive methylation reagents and a glycan-tagging kit termed INLIGHT^® (Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tag) for relative quantitation. Each technique is described and referenced on their associated page.

Dimethyl Labeling

To facilitate reductive amination reactions in quantitative proteome profiling, CIL is pleased to offer a variety of chemical tagging reagents.

INLIGHT^® Kit

To aid the identification and relative quantification of glycans by LC-MS, CIL offers the innovative INLIGHT^® (Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tag) glycan-tagging kit.