Oligonucleotide Synthesis Materials
Stable isotope labeling is a powerful tool utilized in nucleic acid research, enabling detailed investigations of oligonucleotide structure, dynamics and function utilizing methods like nuclear magnetic resonance (NMR) and mass spectrometry (MS). The incorporation of stable isotopes such as 13C, 15N, and 2H into RNA or DNA enables the determination of detailed mechanistic insights and interactions, enhances analytical performance, and even enables quantification. CIL offers a comprehensive product portfolio of high-quality, stable isotope-labeled RNA and DNA building blocks designed for both enzymatic and chemical synthesis approaches.
Two primary strategies are used to introduce stable isotopes into oligonucleotides – enzymatic or synthetic. Enzymatic synthesis involves incorporating labeled nucleoside triphosphates (rNTPs or dNTPs) during in vitro transcription or polymerase reactions and is ideal for uniform or base-specific labeling across the entire oligonucleotide. Chemical synthesis utilizes labeled phosphoramidites in solid-phase synthesis, enabling precise, site-specific placement of isotope labels within the sequence. This method is particularly useful for protein-nucleic acid interactions or structural studies requiring pinpoint accuracy.
Stable Isotope-Labeled Nucleic Acids and Related Compounds